Designing PCR Primers Share: Download MP3 Similar Tracks Retrieving Gene & Promoter Sequences Jacob Elmer Designing gRNA Oligos to Clone into Cas9 Expression Plasmids for KO Experiments Jacob Elmer Real-Time PCR in Action USDA Animal and Plant Health Inspection Service Designing cloning primers for classical (restriction) cloning Katharine Hubbard How to design antisense oligonucleotides for high on-target potency and minimal off-target effect Integrated DNA Technologies (IDT) Sanger Sequencing - Sample Prep & Data Analysis with BLAST Jacob Elmer Understanding PCR Mark Temple Webinar: Nanopore Sequence Data Analysis in CyVerse Discovery Environment – Part 2 CyVerse.org Constructing Plasmids for Cas9/gRNA Expression and Knock Out Experiments Jacob Elmer Miniprep - Plasmid DNA Isolation Jacob Elmer PCR Primer Design, Virtual PCR and Ligation in Benchling Elspeth Smith DNA Transcription (Converting DNA to RNA) Medicosis Perfectionalis PCR Primer Design Biomedical Research Center StatQuest: A gentle introduction to RNA-seq StatQuest with Josh Starmer Agarose Gel Extraction Jacob Elmer SLIC cloning (Sequence and Ligation Independent Cloning) theory & workflow the bumbling biochemist PCR Basics Joseph Ross