Constructing Plasmids for Cas9/gRNA Expression and Knock Out Experiments Share: Download MP3 Similar Tracks Designing gRNA Oligos to Clone into Cas9 Expression Plasmids for KO Experiments Jacob Elmer Designing PCR Primers Jacob Elmer Understanding CRISPR-Cas9 Andrew Douch DNA Replication - Leading Strand vs Lagging Strand & Okazaki Fragments The Organic Chemistry Tutor Retrieving Gene & Promoter Sequences Jacob Elmer CRISPR Cas9 Screening The Ahmed Lab: Northwestern Neurosurgery Real-Time PCR in Action USDA Animal and Plant Health Inspection Service Design high specificity CRISPR Cas9 gRNAs principles and tools GenScript USA Inc. Molecular Biology #1 2020 OLLI UCSC Designing PCR Primers for Restriction Enzyme-mediated Cloning Jacob Elmer Jennifer Doudna: CRISPR Basics Innovative Genomics Institute – IGI Webinar: Nanopore Sequence Data Analysis in CyVerse Discovery Environment – Part 2 CyVerse.org CRISPR Systems: Nature’s Toolkit for Genome Editing - 2018 Dickson Prize Carnegie Mellon University Gene delivery tools webinar | Lentivirus OriGene Technologies Inc. Sanger Sequencing - Sample Prep & Data Analysis with BLAST Jacob Elmer Miniprep - Plasmid DNA Isolation Jacob Elmer Genome editing with the CRISPR-Cas9 system AHAScience Extracting Plasmid DNA: How To Do a Miniprep LabXchange